TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: MARP    
mecA, Molecular Detection, PCR, Varies

Method Description Describes how the test is performed and provides a method-specific reference

Nucleic acids are released from bacterial isolates followed by PCR which employs a target-specific detection system including primers, as well as fluorescent resonance energy transfer (FRET) hybridization probes which target the mecA gene. The LightCycler instrument amplifies and monitors target nucleic acid sequences by fluorescence during PCR cycling. This is an automated PCR system that rapidly detects amplified product development through stringent air-controlled temperature cycling and capillary cuvettes. Detection of amplified products is based on the FRET principle. For FRET product detection, a hybridization probe with a donor fluorophore, fluorescein, on the 3' end is excited by an external light source, which emits light that is absorbed by a second hybridization probe with an acceptor fluorophore. The acceptor fluorophore then emits light of a different wavelength that is measured with a signal proportional to the amount of specific PCR product. The detection process is completed in a closed system.(Cockerill FR, Uhl JR: Applications and challenges of real-time PCR for the clinical microbiology laboratory. In Rapid Cycle Real-Time PCR Methods and Applications. Edited by U Reischl, C Wittwer, F Cockerill. Springer-Verlag, 2002, pp 3-27; Simner PJ, Humphries R: Chapter 75: Special Phenotypic Methods for Detecting Antibacterial Resistance: In Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller. American Society for Microbiology; 2019. pp. 1316-1347)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

5 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester