Test Catalog

Test ID: TBNGS    
Mycobacterium tuberculosis Complex, Molecular Detection of Drug Resistance Markers, Whole Genome Sequencing, Varies

Method Description Describes how the test is performed and provides a method-specific reference

Mycobacterium tuberculosis complex isolates are lysed and genomic nucleic acid is purified and quantified. Library preparation for whole genome sequencing is performed using 50 to 100 ng of the purified M tuberculosis complex DNA and the Illumina TruSeq Nano DNA Library Preparation Kit. Following library preparation, samples are quantitated and pooled on an Illumina sequencer for multiplexed, paired-end whole genome sequencing. Sequences obtained are compared to the M tuberculosis reference genome (strain H37Rv) to identify variants. Variant review and interpretation is completed by comparing the any variants detected with the Mayo Experience Database and any matching variants are reported. A minimum of 40X position coverage, 20X variant coverage, and 15% variant frequency compared to the H37Rv reference genome is required before reporting a variant. Variants of unknown significance are not reported.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Tuesday, Thursday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

7-14 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

14 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 Year

Performing Laboratory Location Indicates the location of the laboratory that performs the test