Web: | mayocliniclabs.com |
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Email: | mcl@mayo.edu |
Telephone: | 800-533-1710 |
International: | +1 855-379-3115 |
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Mycobacterium tuberculosis complex isolates are lysed and genomic nucleic acid is purified and quantified. Library preparation for whole genome sequencing is performed using 50 to 100 ng of the purified M tuberculosis complex DNA and the Illumina TruSeq Nano DNA Library Preparation Kit. Following library preparation, samples are quantitated and pooled on an Illumina sequencer for multiplexed, paired-end whole genome sequencing. Sequences obtained are compared to the M tuberculosis reference genome (strain H37Rv) to identify variants. Variant review and interpretation is completed by comparing the any variants detected with the Mayo Experience Database and any matching variants are reported. A minimum of 40X position coverage, 20X variant coverage, and 15% variant frequency compared to the H37Rv reference genome is required before reporting a variant. Variants of unknown significance are not reported.(Unpublished Mayo method)
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