TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: CVRMP    
Cardiovascular Risk Marker Panel, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Non-HDL Cholesterol:

The non-HDL cholesterol is calculated from serum cholesterol and high-density cholesterol.

Non-HDL=Cholesterol - HDL.(Jacobson TA, Ito MK, Maki KC, et al: National Lipid Association recommendations for patient-centered management of dyslipidemia: part 1 - executive summary. J Clin Lipidol 2014 Sep-Oct;8[5]:473-488)

 

Low-Density Lipoprotein (LDL) Cholesterol:

The LDL cholesterol is calculated from serum cholesterol, serum triglycerides, and high-density cholesterol according to the following formula by Friedewald, et al.(Friedewald WT, Levy RI, Fredrickson DS: Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972 June;18[6]:499-502)

LDL=Cholesterol - HDL - (Triglycerides/5)

 

HDL Cholesterol:

HDL cholesterol is performed by selective precipitation and enzymatic/colorimetric method. In the presence of certain polyanions and certain divalent cations in carefully controlled concentrations, the chylomicrons, very low-density lipoproteins (VLDLs), LDLs, and lipoprotein (a) (Lp[a]) form precipitates, while the HDL remains in solution. Treating a sample of serum with carefully measured amounts of polyanionic (dextran sulfate or heparin) and divalent cationic (calcium or manganese) reagents followed by the measurement of cholesterol in the resulting supernatant fluid provides an accurate determination of the serum concentration of HDL cholesterol. The method is referenced to the Center of Disease Control standardized method.(Package insert: HDL Cholesterol Precipitating Reagent Set [Dextran Sulfate], Pointe Scientific, Canton, MI; package insert: Roche Cholesterol Reagent, Roche Diagnostics Corp, Indianapolis)

 

Cholesterol:

Cholesterol is measured by an automated enzymatic method. The reagents include cholesterol ester hydrolase, cholesterol oxidase, and a coupled colorimetric end-point chemistry system. The method is referenced to the Centers of Disease Control standardized method.(Package insert: Roche Cholesterol Reagent, Roche Diagnostics Corp, Indianapolis)

 

Triglycerides:

Serum triglycerides are measured by an automated enzymatic method. The chemistry includes hydrolysis of the triglycerides and phosphorylation of the resulting glycerol. The method is referenced to the Center of Disease Control standardized method.(Package insert: Roche Triglyceride Reagent, Roche Diagnostics Corp, Indianapolis)

 

Lipoprotein (a) Lp(a):

Serum Lp(a) test uses an automated turbidimetric immunoassay method. Serum is first incubated with a polymeric enhancer. Following initial incubation and measurement of specimen blank, undiluted antiserum specific to human Lp(a) is added. The specimen solution is mixed and insoluble antigen-antibody complexes begin to form. The complexes that form produce turbidity in the mixture and increase the amount of light scatter. The decrease in percent transmittance of light is measured and is proportional to the amount of Lp(a) in the specimen.(Package insert: Roche Tina-quant Lipoprotein (a), Roche Diagnostics Corp, Indianapolis)

 

C-Reactive Protein (CRP):

The quantitative determination of high sensitivity CRP (hs-CRP) is achieved by latex particle-enhanced immunoturbidimetric assay. Latex particles coated with antihuman CRP antibodies aggregate in the presence of serum CRP, forming immune complexes. The formed immune complexes cause increased turbidity, which is proportional to the concentration of CRP in the serum. The sample hs-CRP concentration is determined by comparison to hs-CRP standards of known concentration.(Package insert: Roche Cardiac C-Reactive Protein [Latex] High Sensitive, Roche Diagnostics Corp, Indianapolis)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; Continuously

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester