Test Catalog

Test ID: PDG14    
PDGFRA Exon 14, Mutation Analysis

Method Description Describes how the test is performed and provides a method-specific reference

The paraffin-embedded tissue is macroscopically examined and the tumor-rich portion is dissected, deparaffinized, lysed, and digested. Genomic DNA is extracted using either a phenol-chloroform method or the QIAamp DNA FFPE Tissue kit (Qiagen). The DNA is amplified via PCR. Primers specific for PDGFRA exon 14 are used. Controls are run with each specimen to assess possible contamination issues and overall test performance. The patient and control samples are sent for direct DNA sequencing. The sequencing chromatograms are analyzed by manual and software methods and the presence or absence of a PDGFRA exon 14 mutation is determined. The results are interpreted and reported by a working group pathologist.(Instruction manual: Qiagen DNA FFPE Tissue Handbook; unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

14 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

20 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Unused portions of blocks will be returned. Unused slides are stored indefinitely.

Performing Laboratory Location Indicates the location of the laboratory that performs the test