Web: | mayocliniclabs.com |
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Email: | mcl@mayo.edu |
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Values are valid only on day of printing. |
Under acidic conditions, iron is liberated from transferrin. Lipemic samples are clarified by the detergent. Ascorbate reduces the released Fe(3+) ions to Fe(2+) ions which then react with FerroZine to form a colored complex. The color intensity is directly proportional to the iron concentration and can be measured photometrically.(Package insert: Roche Fe reagent. Indianapolis, IN, 2010)
Anti-transferrin antibodies react with the antigen in the sample to form an antigen/antibody complex. Following agglutination, this is measured turbidimetrically. Addition of polyethylene glycol (PEG) allows the reaction to progress rapidly to the end point and increases sensitivity.(Package insert: Roche TRSF2 reagent. Indianapolis, IN, 2005)
Calculations:
TIBC = (Transferrin x 1.18)
% Saturation = Iron/TIBC x 100
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