Test Catalog

Test ID: HAEVP    
Hemolytic Anemia Evaluation

Useful For Suggests clinical disorders or settings where the test may be helpful

Evaluation of lifelong or inherited hemolytic anemias, including red cell membrane disorders, unstable or abnormal hemoglobin variants, and red cell enzyme disorders


This evaluation is not suitable for acquired causes of hemolysis.

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
HAEVHemolytic Anemia InterpretationNoYes
A2FHemoglobin A2 and FNoYes
HBELHemoglobin Electrophoresis, BNoYes
UNHBUnstable Hemoglobin, BNoYes
FRAGOOsmotic FragilityYes, (Order FRAG)Yes
SCTRLShipping Control VialNoYes
PKPyruvate Kinase, RBCYesYes
GPIGlucose Phosphate Isomerase, BYesYes
HEXKHexokinase, BNoYes
PBSMMorphology ReviewNoYes

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
GLTIGlutathione, BNoNo
SDEXHemoglobin S, Scrn, BYesNo
IEFIEF ConfirmsNoNo
MASSHb Variant by Mass Spec, BNoNo
RBCEReflexed RBC EnzymesNoNo
HPFHHemoglobin F, Red Cell Distrib, BNoNo
ATHALAlpha-Globin Gene AnalysisYesNo
WASQRAlpha Globin Gene Sequencing, BYes, (Order WASEQ)No
WBDDRBeta Globin Cluster Locus Del/Dup,BYes, (Order WBDD)No
HAEVAHemolytic Anemia Summary InterpNoNo
WBSQRBeta Globin Gene Sequencing, BNoNo
WGSQRGamma Globin Full Gene SequencingNoNo

Additional Tests Lists tests that are always performed, at an additional charge, with the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
BND3Band 3 Fluorescence Staining, RBCNoYes

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This is a consultative evaluation in which the case will be evaluated and appropriate tests performed, at an additional charge, and the results interpreted. If a peripheral blood smear is provided, the morphologic features will be incorporated into the interpretation. If a Hemolytic Anemia Patient Information sheet (T705) is received with the sample, the reported clinical features or clinical impression will be incorporated into the interpretation.


The most common RBC enzymes (G6PD,pyruvate kinase, glucose phosphate isomerase, and hexokinase) will always be performed. If these are normal, the second-tier enzymes will be performed (provided sufficient sample volume is available). If second-tier enzymes are desired, even if the first-tier testing is abnormal, fill out the Hemolytic Anemia Patient Information sheet (T705) and indicate this desire. Cation exchange HPLC, capillary electrophoresis, and hemoglobin stability studies will always be performed. Reflex testing required to identify a hemoglobin abnormality can be added as the case requires. Osmotic fragility and eosin-5-maleimide (EMA) binding (band 3) flow cytometry will be performed on all cases. A normal shipping control for osmotic fragility (OF) is necessary to exclude false-positive results due to preanalytical artifact.


OF and EMA binding testing will be canceled if no shipping control is received or if the shipping control is abnormal.


HAEVA / Hemolytic Anemia Summary Interpretation, an additional consultative interpretation that summarizes all testing, will be provided after test completion to incorporate subsequent results into an overall evaluation if 1 or more of the following molecular tests are reflexed on the Hemolytic Anemia Evaluation:

-ATHAL / Alpha-Globin Gene Analysis

-WASQR / Alpha-Globin Gene Sequencing, Blood

-WBSQR / Beta-Globin Gene Sequencing, Blood

-WBDDR / Beta-Globin Cluster Locus Deletion/Duplication, Blood

-WGSQR / Gamma-Globin Full Gene Sequencing


Note: RBCE / Reflexed RBC Enzymes, Blood (second-tier enzymes) includes: adenylate kinase, phosphofructokinase, phosphoglycerate kinase, triosephosphate isomerase, and pyrimidine 5' nucleotidase.


See Benign Hematology Evaluation Comparison in Special Instructions.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Method Name A short description of the method used to perform the test

HAEV: Consultative Interpretation

A2F: Cation Exchange/High-Performance Liquid Chromatography (HPLC)

HBEL: Capillary Electrophoresis

UNHB: Isopropanol and Heat Stability

FRAGO: Osmotic Lysis

G6PD, PK, GPI, HEXK, RBCE, GLTI: Kinetic Spectrophotometry (KS)

PBSM: Consultant Review

BND3 (eosin-5-maleimide (EMA) binding): Flow Cytometry

MASS: Mass Spectrometry (MS)

IEF: Electrophoresis

HPFH: Flow Cytometry

HAEVA: Consultative Interpretation

NY State Available Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name Lists a shorter or abbreviated version of the Published Name for a test

Hemolytic Anemia Evaluation

Aliases Lists additional common names for a test, as an aid in searching

Hemolytic Anemia
EMA binding
Eosin 5 maleimide
RBC membrane
RBC enzyme
Unstable hemoglobin
Pyruvate kinase deficiency
Neonatal anemia
Neonatal hemolysis
Hereditary pyropoikilocytosis
Hereditary spherocytosis