Test Catalog

Test Id : SPN

Pneumocystis Smear, Varies

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis of Pneumocystis jiroveci pneumonia

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
TISSR Tissue Processing No, Bill Only No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex test may be performed at an additional charge.

Method Name
A short description of the method used to perform the test

Calcofluor White Fluorochrome Stain

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Pneumocystis smear

Aliases
Lists additional common names for a test, as an aid in searching

Calcofluor (Question if test not specified)

Pneumocystis carinii smear

Pneumocystis jiroveci smear

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex test may be performed at an additional charge.

Specimen Type
Describes the specimen type validated for testing

Varies

Necessary Information

Specimen source is required.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Bronchoalveolar lavage

Container/Tube: Sterile container

Specimen Volume: Minimum of 2 mL

 

Specimen Type: Lung or open lung tissue

Container/Tube: Sterile container

Specimen Volume: Minimum of a rice size piece

Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.

 

Alternate:

Specimen Type: Bronchial washing, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: Minimum of 1 mL

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Bronchoalveolar lavage: 2 mL

Sputum, bronchial washings, and tracheal secretions: 1 mL

Lung tissue and open lung biopsy: rice-sized piece of tissue

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Fixed tissue
Viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth)
Wood shaft or charcoal swab
Prepared slides, glass slides, microscope slides
Reject
 

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
Ambient 7 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis of Pneumocystis jiroveci pneumonia

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex test may be performed at an additional charge.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Pneumocystis jiroveci is one of the major microbial pathogens associated with opportunistic pulmonary infections in patients receiving immunosuppressive therapy or with immune deficiencies. Presently, the most common means to diagnose Pneumocystis jiroveci infection is by microscopic detection of the organisms in specimens such as bronchoalveolar lavage, open lung biopsy tissue, induced sputum and transtracheal aspirate.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Negative

Interpretation
Provides information to assist in interpretation of the test results

Negative: no cysts observed

Positive: cysts present

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Only the cyst stage of Pneumocystis jiroveci stains by this method

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Olsson M, Elvin K, Linder E, et al: Pneumocystis carinii is still a dangerous opportunist. The infection is continuously a threat to immunocompromised patients. Lakartidningen. 1999 Jan27;96(4):328-331

2. Shelhamer JH, Gill VJ, Quinn TC, et al: The laboratory evaluation of opportunistic pulmonary infections. Ann Intern Med. 1996 Mar 15;124(6):585-599

3. Kim YK, Parulekar S, Yu PK, et al: Evaluation of calcofluor white stain for detection of Pneumocystis carinii. Diagn Microbiol Infect Dis. 1990;13:307-310

4. Baselski VS, Robison MK, Pifer LW, Woods DR: Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining. J Clin Microbiol. 1990;28:393-394

Method Description
Describes how the test is performed and provides a method-specific reference

A rapid Calcofluor white stain is used to detect the cysts of Pneumocystis jiroveci (formerly carinii). Calcofluor white is a nonspecific fluorochrome stain that binds with beta-configuration polysaccharides such as cellulose and chitin. Only the cyst stage of Pneumocystis jiroveci (formerly carinii ) stains by this method. Both the 2 internal structures and cyst wall must be seen before calling the slide positive for Pneumocystis jiroveci.

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 day

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Raw specimen 2-14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87206

87176-Tissue processing (if appropriate)

LOINC® Information

Test Id Test Order Name Order LOINC Value
SPN Pneumocystis smear 48055-8
Result Id Test Result Name Result LOINC Value
Result LOINC Value Tooltip
SPN Pneumocystis smear 48055-8

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports