Test Catalog

Test Id : PAMY

Amylase, Pancreatic, Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

Aiding in the evaluation of acute pancreatitis


This test is not useful for diagnosing or characterizing pancreatic cancer or cysts.

Method Name
A short description of the method used to perform the test

Colorimetric Reaction

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Amylase, Pancreatic, S

Lists additional common names for a test, as an aid in searching

Pancreatic Amylase

P-AMY (Pancreatic Amylase)

Specimen Type
Describes the specimen type validated for testing


Ordering Guidance

For amylase pancreatic cyst or fluid testing, order AMLPC / Amylase, Pancreatic Cyst Fluid.

For amylase testing using other body fluid specimens (eg, peritoneal, pleural), order AMBF / Amylase, Body Fluid.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot 1 mL of serum into plastic vial. Send refrigerated.


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
Frozen 30 days
Ambient 7 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

Aiding in the evaluation of acute pancreatitis


This test is not useful for diagnosing or characterizing pancreatic cancer or cysts.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Amylases degrade complex carbohydrates (starches) into simple sugars. Two isoenzymes, pancreatic and salivary, are found in serum. Serum pancreatic amylase should always be interpreted in a context of total amylase to determine the relative contribution of salivary and pancreatic isoenzymes.


Imaging tests have become the diagnostic tests of choice for diagnosing pancreatitis. Pancreatic isoamylase may be used as an adjunct to totally serum amylase, serum lipase, and imaging tests.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

0-<24 months: 0-20 U/L

2-<18 years: 9-35 U/L

> or =18 years: 13-53 U/L

Provides information to assist in interpretation of the test results

Increased concentrations of pancreatic amylase isoenzymes in a context of elevated total serum amylase may indicate pancreatitis.

Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Elevations of pancreatic amylase may be seen in patients that have macroamylase present. This elevation is caused by the inability of macroamylase to be excreted in the urine and is not diagnostic for pancreatitis, pancreatic cancer or cysts. The presence or absence of macroamylase may be determined by utilizing serum lipase and urinary amylase tests.


Detection of chronic pancreatitis can only be aided by pancreatic amylase during acute episodes.

Icodextrin-based medications may lead to decreased amylase values.


In very rare cases of gammopathy, in particular type IgM (Waldenstrom macroglobulinemia), may cause unreliable results.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

Panteghini M: Laboratory evaluation of pancreatic diseases. Biochimica Clinica. 2010;34(1):19-25

Method Description
Describes how the test is performed and provides a method-specific reference

After immunoinhibition with antibodies against human salivary alpha-amylase the amount of pancreatic alpha-amylase in a sample is selectively determined by an enzymatic colorimetric method using the substrate 4,6-ethylidene-p-nitrophenyl-alpha-D-maltoheptaoside (ethylidene-G7PNP).(Package insert: Alpha-Amylase EPS Pancreatic, Roche Diagnostics; V 13.0. 01/2020)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Saturday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 3 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test


Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.


LOINC® Information

Test Id Test Order Name Order LOINC Value
PAMY Amylase, Pancreatic, S 1805-1
Result Id Test Result Name Result LOINC Value
Result LOINC Value Tooltip
PAMY Amylase, Pancreatic, S 1805-1

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports