Web: | mayocliniclabs.com |
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Email: | mcl@mayo.edu |
Telephone: | 800-533-1710 |
International: | +1 855-379-3115 |
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Molecular detection of drug resistance variants in culture isolates of the Mycobacterium tuberculosis complex
May provide a more rapid detection of drug resistance than phenotypic, broth-based testing
Aiding in the resolution of discrepant results obtained using phenotypic methods testing for M tuberculosis isolates that are not sufficiently viable to allow for culture-based testing
This assay may provide results within 1 week as opposed to phenotypic broth-based or agar-based assays that can take, on average, 14 days to produce results. Additionally, this assay can be useful as an aid in the resolution of discrepant phenotypic results or in instances when the isolate does not grow sufficiently well to allow for phenotypic testing.
Whole genome sequencing (WGS) of Mycobacterium tuberculosis complex isolates is performed followed by evaluation of selected genes of interest for the presence of well-characterized, drug resistance-conferring variants.
Traditional broth-based, phenotypic drug resistance testing should also be performed, since not all genes associated with resistance within the M tuberculosis complex genome have been fully elucidated or are evaluated in this test. If traditional broth-based phenotypic drug resistance testing is desired, add TB1LN / Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, First Line, Varies; TB2LN / Susceptibility, Mycobacterium tuberculosis Complex, Second Line, Varies; and TBPZA / Susceptibility, Mycobacterium tuberculosis Complex, Pyrazinamide, Varies.
Whole Genome Sequencing