Test Catalog

Test Id : TLPF

T-Cell Lymphoma, FISH, Varies

Useful For
Suggests clinical disorders or settings where the test may be helpful

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas

 

Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphoma

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
_I099 Interphases, 25-99 No, Bill Only No
_I300 Interphases, >=100 No, Bill Only No
_IL25 Interphases, <25 No, Bill Only No
_PADD Probe, +1 No, Bill Only No
_PB02 Probe, +2 No, Bill Only No
_PB03 Probe, +3 No, Bill Only No
_PBCT Probe, +2 No, Bill Only No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

 

When this test and flow cytometry testing for leukemia/lymphoma are ordered concurrently, the flow cytometry result will be utilized to determine if sufficient clonal T-cells are available for FISH testing. If the result does not identify a sufficient clonal T-cell population, this FISH test order will be canceled and no charges will be incurred.

 

If FISH testing proceeds, probes will be performed based on the lymphoma subtype suspected/identified utilizing the table located in Clinical Information.

 

If the patient is being tracked for known abnormalities, indicate which probes should be used.

 

The following probe sets are available within the T-cell lymphoma FISH profile:

14q32.1 rearrangement, TCL1A

/i(7q)/ -7/7q-, D7S486/D7Z1

+8, D8Z2/MYC

 

If T-cell prolymphocytic leukemia/lymphoma (T-PLL) is indicated, TCL1A rearrangement is performed, with reflex to the TRAD FISH probe if TCL1A is negative,

 

See Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm in Special Instructions.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

Fluorescence In Situ Hybridization (FISH)

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

T-cell Lymphoma, FISH, B/BM

Aliases
Lists additional common names for a test, as an aid in searching

+8 (trisomy 8)

inv(14) - inversion 14

Iso(7q) - isochromosome 7q

T-cell Prolymphocytic Leukemia (T-PLL)

TCL1A (14q32) rearrangement

Hepatosplenic T-cell Lymphoma

TRAD (14q11.2) rearrangement

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

 

When this test and flow cytometry testing for leukemia/lymphoma are ordered concurrently, the flow cytometry result will be utilized to determine if sufficient clonal T-cells are available for FISH testing. If the result does not identify a sufficient clonal T-cell population, this FISH test order will be canceled and no charges will be incurred.

 

If FISH testing proceeds, probes will be performed based on the lymphoma subtype suspected/identified utilizing the table located in Clinical Information.

 

If the patient is being tracked for known abnormalities, indicate which probes should be used.

 

The following probe sets are available within the T-cell lymphoma FISH profile:

14q32.1 rearrangement, TCL1A

/i(7q)/ -7/7q-, D7S486/D7Z1

+8, D8Z2/MYC

 

If T-cell prolymphocytic leukemia/lymphoma (T-PLL) is indicated, TCL1A rearrangement is performed, with reflex to the TRAD FISH probe if TCL1A is negative,

 

See Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm in Special Instructions.

Specimen Type
Describes the specimen type validated for testing

Varies

Ordering Guidance

This assay detects chromosome abnormalities observed in the blood or bone marrow of patients with T-cell lymphoma.

-For testing paraffin-embedded tissue samples from patients with T-cell lymphoma, see TLYM / T-Cell Lymphoma, FISH, Tissue.

-For patients with T-cell acute lymphoblastic leukemia/lymphoma, order TALLF / T-Cell Acute Lymphoblastic Leukemia [T-ALL], FISH, Varies.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

2. A pathology or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Bone marrow

Container/Tube: Green top (sodium heparin)

Specimen Volume: 1-2 mL

Collection Instructions: Invert several times to mix bone marrow.

 

Acceptable:

Specimen Type: Blood

Container/Tube: Green top (sodium heparin)

Specimen Volume: 7-10 mL

Collection Instructions: Invert several times to mix blood.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Blood: 2 mL

Bone Marrow: 1 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

No specimen should be rejected.

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
Refrigerated

Useful For
Suggests clinical disorders or settings where the test may be helpful

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas

 

Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphoma

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

 

When this test and flow cytometry testing for leukemia/lymphoma are ordered concurrently, the flow cytometry result will be utilized to determine if sufficient clonal T-cells are available for FISH testing. If the result does not identify a sufficient clonal T-cell population, this FISH test order will be canceled and no charges will be incurred.

 

If FISH testing proceeds, probes will be performed based on the lymphoma subtype suspected/identified utilizing the table located in Clinical Information.

 

If the patient is being tracked for known abnormalities, indicate which probes should be used.

 

The following probe sets are available within the T-cell lymphoma FISH profile:

14q32.1 rearrangement, TCL1A

/i(7q)/ -7/7q-, D7S486/D7Z1

+8, D8Z2/MYC

 

If T-cell prolymphocytic leukemia/lymphoma (T-PLL) is indicated, TCL1A rearrangement is performed, with reflex to the TRAD FISH probe if TCL1A is negative,

 

See Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm in Special Instructions.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

T-cell neoplasms are relatively uncommon, accounting for approximately 12% of all non-Hodgkin lymphomas. There are several subtypes of T-cell neoplasms: T-cell acute lymphoblastic leukemia (T-ALL), T-cell prolymphocytic leukemia (T-PLL), T-cell large granular lymphocytic leukemia (T-LGL), anaplastic large cell lymphoma (ALCL), peripheral T-cell lymphoma, and various other cutaneous, nodal, and extranodal lymphoma subtypes.

 

There are a few common chromosome abnormalities associated with specific T-cell lymphoma subtypes evaluated by this FISH test, as follows:

Common Chromosome Abnormalities in T-cell Lymphomas

Lymphoma Subtype

Chromosome Abnormality

FISH Probe

T-cell prolymphocytic leukemia/lymphoma (T-PLL)

inv(14)(q11q32) and t(14;14)(q11;q32)

5'/3'TCL1A

     Reflex: 14q11.2 rearrangement

5'/3'TRAD

Hepatosplenic T-cell lymphoma

Isochromosome 7q

D7S486/D7Z1

Trisomy 8

D8Z2/MYC

These probes have diagnostic relevance and can also be used to track response to therapy.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

An interpretive report will be provided.

Interpretation
Provides information to assist in interpretation of the test results

A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.

 

Detection of an abnormal clone supports a diagnosis of a T-cell lymphoma. The specific abnormality detected may help subtype the neoplasm.

 

The absence of an abnormal clone does not rule out the presence of neoplastic disorder.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

This test is not approved by the U.S. Food and Drug Administration and it is best used as an adjunct to existing clinical and pathologic information.

 

Bone marrow is the preferred sample type for this FISH test. If bone marrow is not available, a blood specimen may be used if there are malignant cells in the blood specimen (as verified by hematopathology).

Supportive Data

Each probe was independently tested on a set of normal bone marrow control samples, and when available bone marrow samples from patients diagnosed with a T-cell lymphoma. Normal cutoffs were calculated based on the results from 25 normal specimens. Each probe set was evaluated to confirm the probe set detected the abnormality it was designed to detect.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Edited by SH Swerdlow, et al. IARC, Lyon 2017

2. Gesk S, Martin-Subero JI, Harder L, et al: Molecular cytogenetic detection of chromosomal breakpoints in T-cell receptor gene loci. Leukemia 2003;17:738-745

3. Chin M, Mugishima H, Takamura M, et al: Hemophagocytic syndrome and hepatosplenic (gamma)(delta) T-cell lymphoma with isochromosome 7q and 8 trisomy. J Pediatr Hematol Oncol 2004;26(6):375-378

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

This test is performed using commercially available and laboratory-developed probes. Rearrangements involving TCL1 and TRAD are detected using a dual-color break-apart (BAP) strategy probe. Trisomy of chromosome 8 and isochromosome 7q are detected using enumeration strategy probes. For each probe set, 200 interphase nuclei are scored and results are expressed as the percent abnormal nuclei.(Unpublished Mayo method)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

7 to 10 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

4 weeks

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

88271x2, 88291-DNA probe, each (first probe set), Interpretation and report

88271x2-DNA probe, each; each additional probe set (if appropriate)

88271x1-DNA probe, each; coverage for sets containing 3 probes (if appropriate)

88271x2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)

88271x3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)

88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)

88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)

88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)

LOINC® Information

Test Id Test Order Name Order LOINC Value
TLPF T-cell Lymphoma, FISH, B/BM In Process
Result Id Test Result Name Result LOINC Value
Result LOINC Value Tooltip
52009 Result Summary 50397-9
52011 Interpretation 69965-2
52010 Result Table 93356-4
54566 Result 62356-1
CG719 Reason for Referral 42349-1
CG720 Specimen 31208-2
52012 Source 31208-2
52013 Method 85069-3
55118 Additional Information 48767-8
53849 Disclaimer 62364-5
52014 Released By 18771-6

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports