Test Catalog

Test ID: HPVHR    
Human Papillomavirus (HPV), High-Risk, DNA In Situ Hybridization

Useful For Suggests clinical disorders or settings where the test may be helpful

Detection of human papillomavirus DNA from high-risk genotypes (16, 18, 31, 33, and 51)

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Human papillomavirus (HPV) High Risk E6/E7 RNA ISH could be performed and reported at the discretion of the Mayo pathologist if clinically indicated but will not be billed separately.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Persistent infections with high-risk human papillomavirus (HPV) genotypes (16, 18, 31, 33, and 51) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.

Interpretation Provides information to assist in interpretation of the test results

This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Belobrov S, Cornall A, Young R, et al: The role of human papillomavirus in p16-positive oral cancers. J Oral Pathol Med. 2018;47(1):18-24

2. Ducatman B: The role of human papillomavirus in oropharyngeal squamous cell carcinoma. Arch Pathol Lab Med. 2018;142(6):715-718

3. Windon M, D'Souza G, Rettig E, et al: Increasing prevalence of human papillomavirus–positive oropharyngeal cancers among older adults. Cancer. 2018;124(14):2993-2999