Test Catalog

Test ID: DBA4    
Hairy Cell Leukemia (DBA44) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Classification of lymphomas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Hairy cell leukemia (DBA.44) antibody recognizes an unknown, fixation-resistant antigen that is expressed in normal mantle zone B-cells. It may be expressed in endothelial cells, monocytoid B cells, and scattered immunoblasts as well. It is characteristically expressed in hairy cell leukemia, as well as a subset of marginal zone lymphomas, and may be useful is classification of these lymphomas.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation; only technical performance of the stain is performed. If an interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Gaman AM: Hairy cell leukemia--a rare type of leukemia. A retrospective study on 39 patients. Rom J Morphol Embryol. 2013;54(3):575-579

2. Jones G, Parry-Jones N, Wilkins B, Else M, Catovsky D, British Committee for Standards in Haematology: Revised guidelines for the diagnosis and malignant of hairy cell leukaemia and hairy cell leukemia variant. Br J Haematol. 2011;156:186-195

3. Sherman MJ, Hanson CA, Hoyer JD: An assessment of the usefulness of immunohistochemical stains in the diagnosis of hairy cell leukemia. Am J Clin Pathol. 2011;136:390-399