Test Catalog

Test ID: HCRC    
Hereditary Colon Cancer Multi-Gene Panel, Varies

Useful For Suggests clinical disorders or settings where the test may be helpful

Providing a comprehensive evaluation for hereditary colon cancer in patients with a personal or family history suggestive of a hereditary colon cancer syndrome


Serving as a second-tier test for patients in whom previous targeted gene variant analyses for specific hereditary colorectal cancer-related genes were negative


Establishing a diagnosis of a hereditary colon cancer syndrome in some cases, allowing for targeted cancer surveillance of associated extra-colonic organs known to be at increased risk for cancer


Identifying variants within genes known to be associated with increased risk for colon cancer allowing for predictive testing of at-risk family members

Genetics Test Information Provides information that may help with selection of the correct genetic test or proper submission of the test request

This test includes next-generation sequencing, Sanger sequencing, array comparative genomic hybridization, and multiplex ligation-dependent probe amplification to evaluate for the genes listed on the panel.


Prior Authorization is available for this assay; see Special Instructions


See Targeted Gene Regions Interrogated by Hereditary Colon Cancer Panel in Special Instructions for details regarding the targeted gene regions identified by this test.

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

The following algorithms are available in Special Instructions:

-Lynch Syndrome Testing Algorithm

-Colonic Polyposis Syndromes Testing Algorithm

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Colorectal cancer occurs in approximately 5% to 6% of individuals in the general population. In rare cases, individuals with a family history of colorectal cancer may be at increased risk for colon and other cancers due to a single-gene predisposition syndrome, known as hereditary colorectal cancer. The 2 most common hereditary colorectal cancer syndromes are Lynch syndrome and familial adenomatous polyposis (FAP). However, there are multiple other genes that are also known to cause to hereditary colorectal cancer or contribute to an increased risk for colorectal cancer. This panel uses next-generation sequencing (NGS), array comparative genomic hybridization (aCGH), and other technologies to evaluate for germline variants in 17 genes known to be associated with an increased risk for colon cancer development. Two of the genes listed, CHEK2 and MLH3, are not associated with a known hereditary cancer syndrome defined by a distinct spectrum of tumors. However, literature suggests that variants in these genes may confer an increased risk for colon cancer and, therefore, are predicted to contribute to cancer risk in patients and families.



Known association


Lynch syndrome


Lynch syndrome


Lynch syndrome


Lynch syndrome


Lynch syndrome


Familial adenomatous polyposis


MYH-associated polyposis


Hereditary mixed polyposis syndrome


Peutz-Jeghers syndrome


Juvenile polyposis syndrome


Juvenile polyposis syndrome


PTEN hamartoma tumor syndrome (ie, Cowden syndrome)


Hereditary diffuse gastric cancer


Oligodontia-colorectal cancer syndrome


Li-Fraumeni syndrome


Low-risk gene


Low-risk gene


Indications for testing include but are not limited to:

-Patients in whom no specific colorectal cancer syndrome is evident but for whom there is a clear familial component

-Patients whose family history is consistent with familial colorectal cancer type X(1)

-Patients with a strong suspicion for a single-gene hereditary colon cancer syndrome based on an autosomal dominant pattern of colon cancer in the family

-Patients with a personal or family history of colonic polyposis

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

An interpretive report will be provided.

Interpretation Provides information to assist in interpretation of the test results

All detected alterations are evaluated according to American College of Medical Genetics and Genomics recommendations.(2) Variants are classified based on known, predicted, or possible pathogenicity and reported with interpretive comments detailing their potential or known significance.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Clinical Correlations:

Some individuals who have involvement of 1 or more of the genes on the panel may have a variant that is not identified by the methods performed (eg, promoter alterations, deep intronic alterations). The absence of a variant, therefore, does not eliminate the possibility of a hereditary colorectal cancer syndrome or other heritable risk for colon cancer. For predictive testing of asymptomatic individuals, it is important to first document the presence of a gene variant in an affected family member.


Test results should be interpreted in context of clinical findings, family history, and other laboratory data. Misinterpretation of results may occur if the information provided is inaccurate or incomplete.


Technical Limitations:

In some cases, DNA variants of undetermined significance may be identified.


Due to the limitations of next-generation sequencing, small deletions and insertions greater than 8 nucleotides in length will not be detected by this test. If a diagnosis of one of the syndromes on this panel is still suspected, consider full gene sequencing using traditional Sanger methods. Single or multi-exon deletions as well as whole gene deletions will be detected by array comparative genomic hybridization (aCGH). 


Rare alterations exist that could lead to false-negative or false-positive results. If results obtained do not match the clinical findings, additional testing should be considered.


In addition to disease-related probes, the multiplex ligation-dependent probe amplification technique utilizes probes localized to other chromosomal regions as internal controls. In certain circumstances, these control probes may detect other diseases or conditions for which this test was not specifically intended. Results of the control probes are not normally reported. However, in cases where clinically relevant information is identified, the ordering physician will be informed of the result and provided with recommendations for any appropriate follow-up testing.


Evaluation Tools:

Multiple in-silico evaluation tools were used to assist in the interpretation of these results. These tools are updated regularly; therefore changes to these algorithms may result in different predictions for a given alteration. Additionally, the predictability of these tools for the determination of pathogenicity is currently not validated.


Unless reported or predicted to cause disease, alterations found deep in the intron or alterations that do not result in an amino acid substitution are not reported. These and common alterations identified for this patient are available upon request.


Reclassification of Variants-Policy:

At this time, it is not standard practice for the laboratory to systematically review likely deleterious alterations or variants of uncertain significance that are detected and reported. The laboratory encourages health care providers to contact the laboratory at any time to learn how the status of a particular variant may have changed over time.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Lindor NM, Rabe K, Petersen GM, et al: Lower cancer incidence in Amsterdam-I criteria families without mismatch repair deficiency: familial colorectal cancer type X. JAMA. 2005;293(16):1979-1985

2. Richards S, Aziz N, Bale S, et al: Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med. 2015 May;17(5):405-424

3. Lindor NM, McMaster ML, Lindor CJ, Greene MH, National Cancer Institute, Division of Cancer Prevention, Community Oncology and Prevention Trials Research Group: Concise handbook of familial cancer susceptibility syndromes-second edition. J Natl Cancer Inst Monogr. 2008;(38):1-93

4. Masciari S, Syngal S: The role of p53 in colorectal cancer. In: Potter JD, Lindor NM, eds. Genetics of Colorectal Cancer. Springer Verlag; 2009:213-217

5. Jaeger E, Leedham S, Lewis A, et al: Hereditary mixed polyposis syndrome is caused by a 40-kb upstream duplication that leads to increased and ectopic expression of the BMP antagonist GREM1. Nat Genet. 2012;44(6):699-703

6. Ligtenberg MJL, Kuiper RP, Chan TL, et al: Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1. Nat Genet. 2009;41(1):112-117

7. Lammi L, Arte S, Somer M, et al: Mutations in AXIN2 cause familial tooth agenesis and predispose to colorectal cancer. Am J Hum Genet. 2004;74:1043-1050

8. Liu HX, Zhou XL, Liu T, et al: The role of hMLH3 in familial colorectal cancer. Cancer Res. 2003;63(8):1894-1899

Special Instructions Library of PDFs including pertinent information and forms related to the test