Test Catalog

Test ID: ABYSR    
Antibody Screen with Reflexed Antibody Identification, RBC

Useful For Suggests clinical disorders or settings where the test may be helpful

Detection of allo- or autoantibodies directed against red blood cell antigens in the settings of pretransfusion testing


Evaluation of transfusion reactions


Evaluation of hemolytic anemia

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If the antibody screen is positive, then antibody identification will be performed.

If the patient has a history of antibodies that are still detected, the antibody screen will be canceled and replaced by the antibody identification.

If certain antibodies are detected and the patient is known to be pregnant, an antibody titration will be performed.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Transfusion and pregnancy are the primary means of sensitization to red cell antigens. In a given population, 2% to 4% of the general population possess irregular red cell alloantibodies. Such antibodies may cause hemolytic disease of the newborn or hemolysis of transfused donor red blood cells.

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


If positive, antibody identification will be performed.

Interpretation Provides information to assist in interpretation of the test results

A positive result (antibody detected) necessitates antibody identification to establish the specificity and clinical significance of the antibody detected.


Alloantibodies detected on pregnant Mayo Clinic-Rochester patients will be evaluated for the allo-antibody titer. If antibody reacts strongly, the titre test will be performed.


Negative results indicate no antibody was detected.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Clinical evaluation of antibodies identified is necessary to determine their potential for harm to the patient at this time and to assess appropriate action to be taken in the future.

Clinical Reference Recommendations for in-depth reading of a clinical nature

AABB Technical Manual. 19th edition. Edited by MK Fung, AF Eder, SL Spitalnik, CM Westhoff: AABB 2017