Test Catalog

Test ID: CATR    
Cold Agglutinin Titer, Serum

Useful For Suggests clinical disorders or settings where the test may be helpful

Detection of cold agglutinins in patients with suspected cold agglutinin disease

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

The cold agglutinin titer test is to be used as a tool in the evaluation of suspected cold agglutinin syndrome. In this syndrome, cold agglutinins, usually IgM with anti-I specificity, attach to the patient's erythrocytes causing a variety of symptoms. Symptoms may include chronic anemia due to premature removal of the sensitized erythrocytes from circulation by hemolysis, to acrocyanosis of the ears, fingers, or toes due to local blood stasis in the skin capillaries.

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


Interpretation Provides information to assist in interpretation of the test results

Patients with cold agglutinin syndrome usually exhibit a titer value greater than 1:512, with rare cases reportedly as low as 1:64.


Normal individuals often have low levels of cold agglutinins.


The test is not a direct measure of clinical significance and must be used in conjunction with other in vitro and in vivo parameters.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Normal individuals may have low levels of cold agglutinins.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Petz LD, Garratty G: Acquired Immune Hemolytic Anemias. New York, Churchill Livingstone, 1980

2. Farratty G, Petz LD, Hoops JK: The correlation of cold agglutinin titrations in saline and albumin with haemolytic anaemia. Br J Haematol 1977;35:587-595